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| Serological identification and molecular characterization of B (A) 02 subtype in patients and blood donors from Eastern China |
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| DOI：10.46701/APJBG.20170117016 |
| KeyWord:B(A)02, serological identification, molecular characterization, gene mutation, rare blood type |
| Author | Institution |
| Wen Wu |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
| Yecui Zhang |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
| Xingying Guo |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
| Peng Wu |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
| Zhibo Zhang |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
| Xiangyan Huang |
Department of Blood Transfusion, The General Hospital of Jinan Military Command, Jinan,Shandong 250031,China. |
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| Abstract: |
| This study was designed to identify the rare?type?ABO?blood?groups, B(A) 02, from Eastern China. Three samples with discordant serological results during routine blood type identification and four samples from one sample’ family were selected. All of them were detected by serological method. The exon 6 and 7 of the ABO genes were amplified by PCR and sequenced. They were typed as AsubB by serology and as BO by genotype. In AsubB samples, nt 700C>G mutation was detected in B gene, which was previously defined as B(A)02 alleles. In these seven samples, six showed B(A)02/O01 and one showed B(A)02/O02.B(A)02 allele was found to be more common in this study than B(A)04 which is considered to be more frequent than B(A)02. The careful identification of rare blood types is important for the safety of clinical blood transfusion. |
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