One simple and efficient technical method of total RNA extraction
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KeyWord:RNA extraction, quality, ethanol
Wei Jiang Medical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, China.
Lizhou Jia Key Laboratory of Antibody Technology, National Health and Family Planning Commission, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Gradimir N. Misevic Laboratory of Bioenergetics/Microbiology, University of Geneva, Switzerland.
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      In this article, a convenient and efficient approach has been developed to isolate total RNA from cells. Conventional RNA isolation protocol is time-consuming, so to improve this, a modified method was introduced to eliminate ethanol residue by using a tip complex during the process of RNA extraction. It saves time by isolating RNA in this way and achieves high yields. Additionally, the quality of RNA is guaranteed and verified to be applicable for PCR, cDNA synthesis and reverse transcription PCR.