Serological and molecular analysis of anti-Tja: Case report
  
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KeyWord:anti-Tja, P blood group, p phenotype, A4GALT
                    
AuthorInstitution
Hiyuan Xu Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China
Haochun Chang Nobel Prize Winner Erwin Neher Research Institute, Jiangsu Zhongji Wantai Biological Pharmaceutical Co., Ltd. Jiangyin, Jiangsu 214400, China; Department of Clinical Medicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China; The United Reference Laboratory for Blood Group Gene Detection, National Center for Clinical Laboratories, Beijing 100730, China
Xiaojie Zhu Nobel Prize Winner Erwin Neher Research Institute, Jiangsu Zhongji Wantai Biological Pharmaceutical Co., Ltd. Jiangyin, Jiangsu 214400, China; The United Reference Laboratory for Blood Group Gene Detection, National Center for Clinical Laboratories, Beijing 100730, China
Chen Cao Nobel Prize Winner Erwin Neher Research Institute, Jiangsu Zhongji Wantai Biological Pharmaceutical Co., Ltd. Jiangyin, Jiangsu 214400, China; The United Reference Laboratory for Blood Group Gene Detection, National Center for Clinical Laboratories, Beijing 100730, China
Tianhong Miao Blood Group Lab, Beijing Red Cross Blood Center, Beijing 100088, China
Jiwu Gong The United Reference Laboratory for Blood Group Gene Detection, National Center for Clinical Laboratories, Beijing 100730, China;Department of Transfusion, National Center of Gerontology, Beijing Hospital, Beijing 100730, China
Yu-Shiang Lin Nobel Prize Winner Erwin Neher Research Institute, Jiangsu Zhongji Wantai Biological Pharmaceutical Co., Ltd. Jiangyin, Jiangsu 214400, China; The United Reference Laboratory for Blood Group Gene Detection, National Center for Clinical Laboratories, Beijing 100730, China
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Abstract:
      P antigen frequency is very low in the Chinese population. However, the presence of anti-P1PPk (anti-Tja) is a huge risk for patients undergoing clinical transfusions and recurrent abortions. This report aims to describe p antigen and anti-Tja serological test features and suggests ways in which we may better identify the p antigen. Polymerase chain reaction was used to amplify A4GALT and B3GALNT1, which were then analysed for polymorphisms using Sanger sequencing. The A4GALT sequence results revealed c. 547-548delAT (HE818933), which resulted in a frame shift at aa 183 stopping at aa 281 (M183fs, 281X). Compared with the reference sequence, B3GALNT1 did not show any variations in any of the subjects assessed. Eggs from Columba livia were used in the neutralised P substance test, but failed to neutralise anti-Tja. The serological test and molecular analysis confirmed that the P blood antigens are caused by A4GALTc. 547-548 AT deletion, and the neutralised P substance test cannot identify anti-PP1Pk from RBC alloantibodies against high frequency antigens.
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