Optimization and performance validation for the fully automatic blood type analysis system
  
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KeyWord:blood type, performance validation, parameters
                 
AuthorInstitution
Rongrong Pang Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
Libo Zhang Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
Yong Wang Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
Wenping Han Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
Min Huang Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
Qiang Fu Nanjing Red Cross Blood Center, Nanjing, Jiangsu 210003, China
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Abstract:
      To evaluate and validate the application of fully automatic blood type analysis system parameters under actual lab conditions. All key system parameters were optimized and validated accordingly. The optimized parameters were centrifugal speed at 550 rpm; centrifugal time 20 min; resuspension speed 1,200 rpm; resuspension time: 45 s; incubation temperature 25℃ ; incubation time 400 s; incubation rate: 0 rpm. The sampled red blood cell concentration was 3%. The ratio of plasma to red blood cells reagent was 60 μL:30 μL; the ratio of antibody (reagent) to sampled diluted red blood cell was 30 μL:30 μL. After applying our key parameters for optimization and validation, the automatic blood type detection system's performance was found to meet the relevant requirements, effectively improving the accuracy and reliability of the detection system.
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