Serological and genetic analysis of ABO ambiguous blood group samples
  
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DOI:10.46701/BG.2020022020112
KeyWord:ABO blood group, PCR-SSP, gene sequencing
                    
AuthorInstitution
Ying Li Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Jinming Xu Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Yongliang Zhu Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Ting Xiong Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Ling Zhao Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Yun Yao Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Xiaoyu Zhou Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
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Abstract:
      The accuracy of regular serum methods to detect ABO blood groups can be negatively affected by some factors, such as irregular antibodies, autoantibodies or effects of diseases leading to false or weak agglutination. This study aimed to accurately identify ambiguous ABO blood groups by serological and gene detection methods. The samples were collected in the First Affiliated Hospital of Nanjing Medical University from December 2018 to December 2019. ABO genotyping was performed by polymerase chain reaction-sequence specific primer (PCR-SSP) method in 20 samples, and ABO exons 6 and 7 or FUT1 and FUT2 genes were sequenced in 5 samples. The genes detected in the 21 specimens included 4 cases of A/B, 2 cases of A205/O01, 3 cases of A/O01, 3 cases of A/O02, 1 case of O01/O01, 1 case of O01/O02, 1 case of B/O01, 1 case of B/O02, 1 case of Bel/O01, 1 case of Cisab01/O01, 1 case of rare B/O04, 1 case of Bombay-like Bmh, 1 case of new gene showing c.261del G of exon 6, c.579 T>C of exon 7 and B new/O01. This study suggests that ABO blood group genotyping technology combined with serological typing can be used for accurately typing ambiguous blood groups.
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